Publications

2024

M. Logarušić, K. Šubar, M. Nikolić, A. Jurinjak Tušek, A. Damjanović, M. Radović, I. Radojčić Redovniković, P. Žnidaršič-Plazl, W. Kroutil, M. Cvjetko Bubalo. Harnessing the potential of deep eutectic solvents in biocatalysis: design strategies using CO₂ to formate reduction as a case study. Front. Chem. 2024, 12, 1467810. doi: 10.3389/fchem.2024.1467810

A. Ramšak, M. Gazvoda, I. Plazl, R. Ambrožič, Cu-alginate hydrogels in microfluidic systems: a sustainable catalytic approach for click chemistry. Front. Chem. Eng., 2024, 6, 1434131, doi: 10.3389/fceng.2024.1434131

An interview with Prof. Polona Žnidaršič Plazl. Chem. Eng. Processing – Process Intensification, 2024, . https://doi.org/10.1016/j.cep.2024.109909.

M. Seručnik, I. Dogsa, L.J. Zadravec, I. Mandic-Mulec, P. Žnidaršič-Plazl. Development of a microbioreactor for Bacillus subtilis biofilm cultivation. Micromachines, 2024, 24, 15, 1037. doi: 10.3390/mi15081037.

B. Šketa, J. L. Galman, N. J. Turner, P. Žnidaršič-Plazl. Immobilization of His₆-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes. New Biotechnol. 2024, 83, 46–55, doi:10.1016/j.nbt.2024.06.005.

T. Menegatti, T. Kopač, P. Žnidaršič-Plazl. Tuning mechanical characteristics and permeability of alginate hydrogel by polyvinyl alcohol and deep eutectic solvent addition. Bioengineering, 2024, 11 (4), 371, doi: 10.3390/bioengineering11040371. https://www.mdpi.com/2306-5354/11/4/371,

R. Ambrožič, U. Krühne, I. Plazl. Microfluidics with redox-responsive hydrogels for on-demand BPA degradation. Chem. Eng. J., 2024, 485, 149542, doi: 10.1016/j.cej.2024.149542 https://www.sciencedirect.com/science/article/pii/S1385894724010271, https://repozitorij.uni-lj.si/IzpisGradiva.php?id=155768

T. Menegatti, I. Plazl, P. Žnidaršič-Plazl. Model-based design of continuous biotransformation in a microscale bioreactor with yeast cells immobilized in a hydrogel film. Chem. Eng. J., 2024, 483, 149317, doi: 10.1016/j.cej.2024.149317. https://www.sciencedirect.com/science/article/abs/pii/S1385894724008027, https://repozitorij.uni-lj.si/IzpisGradiva.php?id=154519

2023

T. Menegatti, Ž. Lavrič, P. Žnidaršič-Plazl. Microfluidics-based preparation of cross-linked enzyme aggregates. WO2023175002A1

G. Koplányi, E. Bell, Z. Molnár, G. Katona, P.L. Neumann, F. Ender, G.T. Balogh, P. Žnidaršič-Plazl, L. Poppe, D. Balogh- Weiser. Novel approach for isolation and immobilization of a recombinant transaminase applying an advanced nanocomposite system. ChemBioChem 2023, e202200713, doi: 10.1002/cbic.202200713

2022

A. Zeko-Pivać, M. Tišma, P. Žnidaršič-Plazl, B. Kulisic, G. Sakellaris, J. Hao, M. Planinić. The potential of brewer’s spent grain in the circular bioeconomy : state of the art and future perspectives. Front. Bioeng. Biotechnol., 2022, 10, 1-15, doi: 10.3389/fbioe.2022.870744.

M. Ružič, J. Svetelj, S. Zupančič, I. Plazl, P. Žnidaršič Plazl, M. Bitenc, B. Regina. A continuous process for the preparation of (S)-methyl N((2′-cyano-[1,1′-biphenyl]-4-yl)methyl)-N-pentanoylvalinate in a flow reactor. European patent application EP 3 939 967 A1, 2022-01-19. München: European Patent Office, 2022. 22 p.

2021

R. Ambrožič, I. Plazl. Development of an electrically responsive hydrogel for programmable in situ immobilization within a microfluidic device. Soft Matter, 2021, 17, 6751 – 6764, doi: 10.1039/d1sm00510c

T. Menegatti, P. Žnidaršič-Plazl. Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactor. Front. Bioeng. Biotechnol., 2021, 9, 752064, doi: 10.3389/fbioe.2021.752064.

P. Žnidaršič-Plazl. Biocatalytic process intensification via efficient biocatalyst immobilization, miniaturization, and process integration. Curr. Opin. Green Sustain. Chem., 2021, 32, 100546, doi: 10.1016/j.cogsc.2021.100546.

M. Tišma, P. Žnidaršič-Plazl, G. Šelo, I. Tolj, M. Šperanda, A. Bucić-Kojić, M. Planinić. Trametes versicolor in lignocellulose-based bioeconomy : state of the art, challenges and opportunities. Bioresour. Technol., 2021, 330, 124997, doi: 10.1016/j.biortech.2021.124997.

G.N. Jovanovic, M. Coblyn, I. Plazl. Time scale analysis & characteristic times in microscale-based chemical and biochemical processes. Part 1, Concepts and origins. Chem. Eng. Sci., 2021, 238, 116502, doi: 10.1016/j.ces.2021.116502.

G.N. Jovanovic, M. Coblyn, I. Plazl. Time scale analysis & characteristic times in microscale-based chemical and biochemical processes. Part 2, Bioreactors with immobilized cells, and process flowsheet analysis. Chem. Eng. Sci., 2021, 236, 116499, doi: 10.1016/j.ces.2021.116499.

F. Strniša, T. V. Sagar, P. Djinović, A. Pintar, I. Plazl. Ni-containing CeO₂ rods for dry reforming of methane: activity tests and a multiscale lattice Boltzmann model analysis in two model geometries. Chem. Eng. J., 2021, 413, 127498, doi: 10.1016/j.cej.2020.127498.

D.K. Martin, O. Vicente, T. Beccari, M. Kellermayer, M. Koller, R. Lal, R.S. Marks, I. Marova, A. Mechler, D. Tapaloaga, P. Žnidaršič-Plazl, M. Dundar. A brief overview of global biotechnology. Biotechnol. Biotechnol. Equip., 2021, 35 (1), 354-363, doi: 10.1080/13102818.2021.1878933

P. Žnidaršič-Plazl. Let the Biocatalyst Flow. Acta Chim Slov., 2021, 68, 1-16, doi:10.17344/acsi.2020.6488

A. Hubman, I. Plazl, T. Urbič. Inertial focusing of neutrally buoyant particles in heterogenous suspensions. J. Mol. Liq., 2021, 328, 115410, doi: 10.1016/j.molliq.2021.115410

2020

M. Seručnik, F. A. Vicente, Ž. Brečko, J.A.P. Coutinho, S.P.M. Ventura, P. Žnidaršič-Plazl. Development of a Microfluidic Platform for R‑Phycoerythrin Purification Using an Aqueous Micellar Two-Phase System. ACS Sustainable Chem. Eng., 2020, 8 (46), 17097-17105, doi: 10.1021/acssuschemeng.0c05042

F. Strniša, P. Žnidaršič-Plazl, I. Plazl. Lattice Boltzmann modeling-based design of a membrane-free liquid-liquid microseparator. Chem. Biochem. Eng. Q., 2020, 34, 73-78, doi: 10.15255/CABEQ.2020.1781.

F. A. Vicente, I. Plazl, S.P.M. Ventura, P. Žnidaršič-Plazl. Separation and purification of biomacromolecules based on microfluidics. Green Chem., 2020, 22, 4391-4410, doi: 10.1039/c9gc04362d.

F. Strniša, T. Urbič, I. Plazl. A lattice Boltzmann study of 2D steady and unsteady flows around a confined cylinder. J. Braz. Soc. Mech. Sci. & Eng., 2020, 42 (2), 1-13, doi: 1007/s40430-020-2176-y

G. Stojkovič, P. Žnidaršič-Plazl. Covalent Immobilization of Microbial Cells on Microchannel Surfaces. In: Guisan J., Bolivar J., López-Gallego F., Rocha-Martín J. (Eds.) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 2100. Humana Press, New York, NY, 2020, pp. 417-426, doi: 10.1007/978-1-0716-0215-7_28

2019

T. Menegatti, P. Žnidaršič-Plazl. Copolymeric hydrogel-based immobilization of yeast cells for continuous biotransformation of fumaric acid in a microreactor. Micromachines, 2019, 10, 867; doi: 10.3390/mi10120867

P. Žnidaršič-Plazl. The promises and the challenges of biotransformations in micro-flow. Biotechnol. J., 2019, 14, 1800580, doi: 10.1002/biot.201800580.

F. Strniša, T. Urbič, P. Žnidaršič-Plazl, I. Plazl Process intensification and miniaturization of chemical and biochemical processes. Comput. Aided Chem. Eng., 2019, 1801-1806, doi: 10.1016/B978-0-12-818634-3.50301-5

2018

F. Strniša, M. Bajić, P. Panjan, I. Plazl, A.M. Sesay, P. Žnidaršič-Plazl. Characterization of an enzymatic packed-bed microreactor : Experiments and modeling. Chem. Eng. J, 2018, 350, 541-550, doi: 10.1016/j.cej.2018.05.028.

N. Miložič, G. Stojkovič, A. Vogel, D. Bouwes, P. Žnidaršič-Plazl. Development of microreactors with surface-immobilized biocatalysts for continuous transamination. New Biotechnol., 2018, 47, 18-24, doi: 10.1016/j.nbt.2018.05.004.

M. Bajić, P. Žnidaršič-Plazl, M. Kingston, V. Hessel. General aspects of immobilized biocatalysts and their applications in flow. In: M. B. Nielsen (Ed.). Science of Synthesis: Knowledge Updates 2018/1. Stuttgart; New York: Georg Thieme. 2018, pp. 397-443, doi: 10.1055/sos-SD-216-00409

2017

P. Žnidaršič-Plazl, I. Plazl. Microbioreactors. In: M. Moo-Young, C. Webb (Eds.) Comprehensive Biotechnology, Vol. 2, Engineering perspectives in biotechnology. 3^rd Ed. Amsterdam; Oxford; Cambridge (MA): Elsevier: Pergamon, 2017, pp 414-427, ISBN 978-0-444-64047-5, ISBN 978-0-444-64046-8, doi: 10.1016/B978-0-12-809633-8.09071-3

P. Žnidaršič-Plazl. Biotransformations in microflow systems: Bridging the gap between academia and industry. J Flow Chem., 2017, 7 (3-4), 111-117, doi: 10.1556/1846.2017.00021

N. Miložič, M. Lubej, M. Lakner, P. Žnidaršič-Plazl, I. Plazl. Theoretical and experimental study of enzyme kinetics in a microreactor system with surface-immobilized biocatalyst. Chem. Eng. J., 2017, 313, 374-381, doi: 10.1016/j.cej.2016.12.030.

M. Bajić, I. Plazl, R. Stloukal, P. Žnidaršič-Plazl. Development of a miniaturized packed bed reactor with ω-transaminase immobilized in LentiKats®. Process Biochem., 2017, 52, 63-72, doi: 10.1016/j.procbio.2016.09.021.

A. Prašnikar, T. Urbič, I. Plazl. Microscale technology and biocatalytic processes. Part 1, How to minimize the gap between research and industry. Chim. Oggi – Chem. Today, 2017, 35 (3),18-20, http://www.teknoscienze. com/tks_article/microscale-technology-and-biocatalytic-processes-part-1-how-to-minimize-the-gap-between-research-and-industry/

A. Prašnikar, T. Urbič, I. Plazl. Microscale technology and biocatalytic processes. Part 2, Scale-up case of surface enzyme-catalyzed biotransformation. Chim. Oggi – Chem. Today, 2017, 35 (4), 12-14, http://www.teknoscienze. com/tks_article/microscale-technology-and-biocatalytic-processes-part-2-scale-up-case-of-surface-enzyme-catalyzed-biotransformation/.

2016

U. Novak, D. Lavric, P. Žnidaršič-Plazl. Continuous lipase B-catalyzed isoamyl acetate synthesis in a two-liquid phase system using Corning® AFR TM module coupled with a membrane separator enabling biocatalyst recycle. J. Flow Chem., 2016, 6, 33-38, doi: 10.1556/1846.2015.00038,

P. Žnidaršič-Plazl, B. Zelić. Implementation of microreactor technology in biotechnology : IMTB 2015 conference (editorial). J. Flow Chem., 2016, 6, 1-2, doi: 10.1556/1846.2016.00007

I. Pribec, T. Urbič, I. Plazl. Modelling reaction-diffusion dynamics in microsystems. In: Z. Kravanja, M. Bogataj, (Eds.) 26^th European Symposium on Computer Aided Process Engineering. Amsterdam; Oxford; Cambridge, MA: Elsevier, 2016, 38, 1623-1628, doi: 10.1016/B978-0-444-63428-3.50275-7

2015

R. Wohlgemuth, I. Plazl, P. Žnidaršič-Plazl, K. V. Gernaey, J. M. Woodley. Microscale technology and biocatalytic processes: opportunities and challenges for synthesis. Trends Biotechnol., 2015, 33, 302-314, doi: 10.1016/j.tibtech.2015.02.010

G.N. Jovanovic, J. E. Atwater, P. Žnidaršič-Plazl, I. Plazl. Dechlorination of Polychlorinated Phenols on Bimetallic Pd/Fe Catalyst in a Magnetically Stabilized Fluidized Bed. Chem. Eng.J., 2015, 274, 50-60, doi: 10.1016/j.cej.2015.03.087

M. Lubej, U. Novak, M. Liu, M. Martelanc, M. Franko, I. Plazl. Microfluidic droplet-based liquid-liquid extraction: online model validation, Lab Chip, 2015, 15, 2233-2239, doi: 10.1039/C4LC01460J

U. Novak, M. Lakner, I. Plazl, P. Žnidaršič-Plazl. Experimental studies and modeling of α-amylase aqueous two-phase extraction within a microfluidic device. Microfluid. Nanofluid., 2015, 19, 75-83, doi: 10.1007/s10404-015-1550-z

2014

G. Stojkovič, M. Krivec, A. Vesel, M. Marinšek, P. Žnidaršič-Plazl. Surface cell immobilization within perfluoroalkoxy microchannels. Appl. Surf. Sci., 2014, 320, 810-817, doi: 10.1016/j.apsusc.2014.09.064.

P. Žnidaršič-Plazl, B. Zelić. Implementation of Microreactor Technology in Biotechnology IMTB Conference : a new platform for an interdisciplinary science and technology : editorial. Chem. Biochem. Eng. Q., 2014, 28, 1-2. https://hrcak.srce.hr/120548

N. Miložič, M. Lubej, U. Novak, P. Žnidaršič-Plazl, I. Plazl. Evaluation of diffusion coefficient determination using a microfluidic device. Chem. Biochem. Eng. Q., 2014, 28, 215-223, doi: 10.15255/CABEQ.2014.1938.

M. Lubej, I. Plazl. Theoretical and experimental study of iron catalyst preparation by chemical vapor deposition of ferrocene in air. Chem. Eng. J., 2014, 242, 306-312, doi: 10.1016/j.cej.2013.12.038.

P. Žnidaršič-Plazl. Enzymatic microreactors utilizing non-aqueous media. Chimica Oggi – Chem. Today, 2014, 32, 54-61, https://www.teknoscienze.com/tks_article/enzymatic-microreactors-utilizing-non-aqueous-media/

2013

B. Skubic, M. Lakner, I. Plazl. Sintering behavior of expanded perlite thermal insulation board : modeling and experiments. Ind. Eng. Chem. Res., 2013, 52, 10244-10249, doi: 10.1021/ie400196z.

M. Ilu, U. Novak, I. Plazl, M. Franko. Optimization of a thermal lens microscope for detection in a microfluidic chip. Int. J. Thermophysics, 2013, doi: 10.1007/s10765-013-1515-y.

P. Žnidaršič-Plazl, B. Zelić. Implementation of Microreactor Technology in Biotechnology (IMTB) 2013 Conference : a new community for new perspectives. (editorial) Green Proc. Synth, 2013, 2, 537-539, doi: 10.1515/gps-2013-0101.

B. Ungerböck, A. Pohar, T. Mayr, I. Plazl. Online oxygen measurements inside a microreactor with modeling of transport phenomena. Microfluid Nanofluid., 2013, 14, 565-574, doi: 10.1007/s10404-012-1074-8.

U. Novak, P. Žnidaršič-Plazl. Integrated lipase-catalyzed isoamyl acetate synthesis in a miniaturized system with enzyme and ionic liquid recycle. Green Proc Synth., 2013, 2, 561-568, doi: 10.1515/gps-2013-0082

M. Cvjetko Bubalo, I. Sabotin, I. Radoš, J. Valentičič, T. Bosiljkov, M. Brnčić, P. Žnidaršič-Plazl. A comparative study of ultrasound-, microwave-, and microreactor-assisted imidazolium-based ionic liquid synthesis. Green Proc. Synth., 2013, 2, 579-590, doi: 10.1515/gps-2013-0086

2012

M. Cvjetko, J. Vorkapić-Furač, P. Žnidaršič-Plazl. Isoamyl acetate synthesis in imidazolium-based ionic liquids using packed bed enzyme microreactor. Process Biochem., 2012, 47, 1344-1350, doi: 10.1016/j.procbio.2012.04.028.

G. Stojkovič, P. Žnidaršič-Plazl. Continuous synthesis of L-malic acid using whole-cell microreactor. Process Biochem., 2012, 47, 1102-1107, doi: 10.1016/j.procbio.2012.03.023

U. Novak, A. Pohar, I. Plazl, P. Žnidaršič-Plazl. Ionic liquid-based aqueous two-phase extraction within a microchannel system. Sep. Purif. Technol., 2012, 97, 172–178, doi: 10.1016/j.seppur.2012.01.033.

A. Pohar, M. Lakner, I. Plazl. Parallel flow of immiscible liquids in a microreactor : modeling and experimental study. Microfluid. Nanofluid., 2012, 12, 307-316, doi: 10.1007/s10404-011-0873-7.

M.P.C. Marques, P. Fernandes, J.M.S. Cabral, P. Žnidaršič-Plazl, I. Plazl. Continuous steroid biotransformations in microchannel reactors. New Biotechnol., 2012, 29, 227-234, doi: 10.1016/j.nbt.2011.10.001.

A. Pohar, P. Žnidaršič-Plazl, I. Plazl. Integrated system of a microbioreactor and a miniaturized continuous separator for enzyme catalyzed reactions. Chem. Eng. J., 2012, 189–190, 376–382, doi: 10.1016/j.cej.2012.02.035

2011

P. Žnidaršič-Plazl, I. Plazl. Microbiorectors. In: M. Moo-Young (Ed.-in Chief), Comprehensive Biotechnology, 2^nd Ed., Amsterdam: Elsevier, 2011, pp. 289-301, https://www.sciencedirect.com/referencework/9780080885049/comprehensive-biotechnology

G. Stojkovič, I. Plazl, P. Žnidaršič-Plazl. L-Malic acid production within a microreactor with surface immobilised fumarase. Microfluid. Nanofluid., 2011, 10, 627-635, doi: 10.1007/s10404-010-0696-y.

M. Cvjetko, P. Žnidaršič-Plazl. Ionic liquids within microfluidic devices. In: A. Kokorin (Ed.), Ionic liquids: theory, properties, new approaches. Rijeka: Intech, 2011, 681-700, http://www.intechopen.com/books/ionic-liquids-theory-properties-new-approaches/ionic-liquids-within-microfluidic-devices.

2010

I. Plazl, M. Lakner. Modeling and finite difference numerical analysis of reaction-diffusion dynamics in a microreactor. Acta Chim. Slov, 2010, 57, 100-109, http://acta.chem-soc.si/57/57-1-100.pdf.

M. Tišma, P. Žnidaršič-Plazl, I. Plazl, Đ. Vasić-Rački, B. Zelić. Oxidation of coniferyl alcohol catalyzed by laccases from Trametes versicolor. Acta Chim. Slov. 2010, 57, 110-117, http://acta.chem-soc.si/57/57-1-110.pdf.

P. Žnidaršič-Plazl, I. Plazl. Development of a continuous steroid biotransformation process and product extraction within microchannel system. Catal. Today, 2010, 157, 315-320, doi: 10.1016/j.cattod.2010.01.042.

M.P.C. Marques, P. Fernandes, J.M.S. Cabral, P. Žnidaršič-Plazl, I. Plazl. On the feasibility of in-situ steroid biotransformation and product recovery in microchannels. Chem. Eng. J., 2010, 160, 708-714, doi: 10.1016/j.cej.2010.03.056.

G. Stojkovič, P. Žnidaršič-Plazl. Immobilization of yeast cells within microchannels of different materials. Acta Chim. Slov., 2010, 57, 144-149, http://acta.chem-soc.si/57/57-1-144.pdf.

P. Žnidaršič-Plazl. Mikroreaktorska tehnologija: še ena prelomnica v znanosti in tehnologiji. Delo (Ljubl.), 18. 11. 2010, 52: 24.

2009

A. Pohar, I. Plazl. Process intensification through microreactor application. Chem. Biochem. Eng. Q., 2009, 23, 537-544, http://www.old.pbf.hr/cabeq/pdf/23_4_2009/Cabeq%202009-04-17.pdf.

M. Tišma, B. Zelić, Đ. Vasić-Rački, P. Žnidaršič-Plazl, I. Plazl. Modelling of laccase-catalyzed L-DOPA oxidation in a microreactor. Chem. Eng. J., 2009, 149, 383-388, doi: 10.1016/j.cej.2009.01.025.

A. Pohar, I. Plazl, P. Žnidaršič-Plazl. Lipase-catalyzed synthesis of isoamyl acetate in an ionic liquid/n-heptane two-phase system at the microreactor scale. Lab Chip, 2009, 9, 3385-3390, doi: 10.1039/b915151f.

P. Žnidaršič-Plazl, I. Plazl. Modelling and experimental studies on lipase-catalyzed isoamyl acetate synthesis in a microreactor. Process Biochem., 2009, 44, 1115-1121, doi: 10.1016/j.procbio.2009.06.003.

2008

M. Lakner, I. Plazl. The finite differences method for solving systems on irregular shapes. Comput. Chem. Eng.. 2008, 32, 2891-2896, doi:10.1016/j.compchemeng.2008.02.005.

A. Pohar, I. Plazl. Laminar to turbulent transition and heat transfer in a microreactor : mathematical modeling and experiments. Ind. Eng. Chem. Res., 2008, 47, 7447-7455, doi: 10.1021/ie8001765.

2007

P. Žnidaršič-Plazl, I. Plazl. Steroid extraction in a microchannel system: mathematical modelling and experiments. Lab Chip, 2007, 7, 883-889, doi: 10.1039/B704432A

2005

G. Jovanović, P. Žnidaršič-Plazl, P. Sakrittichai, K. Al-Khaldi. Dechlorination of p-chlorophenol in a microreactor with bimetallic Pd/Fe catalyst. Ind. Eng. Chem. Res.. 2005, 44, 5099-5106, doi: 10.1021/ie049496%2B.